A genetic replacement system for selection-based engineering of essential proteins
نویسندگان
چکیده
منابع مشابه
A genetic replacement system for selection-based engineering of essential proteins
BACKGROUND Essential genes represent the core of biological functions required for viability. Molecular understanding of essentiality as well as design of synthetic cellular systems includes the engineering of essential proteins. An impediment to this effort is the lack of growth-based selection systems suitable for directed evolution approaches. RESULTS We established a simple strategy for g...
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ژورنال
عنوان ژورنال: Microbial Cell Factories
سال: 2012
ISSN: 1475-2859
DOI: 10.1186/1475-2859-11-110